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Fig. 1 | BMC Cancer

Fig. 1

From: Malignant canine mammary epithelial cells shed exosomes containing differentially expressed microRNA that regulate oncogenic networks

Fig. 1

Characterization of putative exosomes. a Representative transmission electron micrograph (TEM) from ultracentrifuge-purified extracellular vesicles. Extracellular vesicles were irregularly round and varied widely in diameter from approximately 50–100 nm. Scale bar = 250 nm. b Representative intensity-weighted dynamic light scattering curve for ultracentrifuge-derived extracellular vesicle fractions. The mean intensity-weighted diameter of microvesicles varied from approximately 150–200 nm. The small peak between 1000 and 10,000 nm likely represents large polydisperse aggregates of particles. c Western blot of CD9 protein demonstrated both CMT and CMEC samples are positively immunoreactive for a protein approximately 20–25 kD in size. The CMT sample was more intensely positive despite the same total protein input, suggesting higher exosomal content in the CMT conditioned media

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