Simultaneous detection of lung fusions using a multiplex RT-PCR next generation sequencing-based approach: a multi-institutional research study

Table 5 Discordant ALK clinical samples

Sample	Laboratory	FISH (rearranged cells)	AmpliSeq result (n° reads)	3′-5′ imbalance	IHC results
26	Viollier	Positive (19%)	Negative (136146)	0,0099	Positive
30	CROM	Positive (20%)	Negative (166403)	0,0008	2+
107	Queen’s	Negative	EML4(6a)-ALK(20) (1835387)	0,3179	Positive

ISSN: 1471-2407