Simultaneous detection of lung fusions using a multiplex RT-PCR next generation sequencing-based approach: a multi-institutional research study

Table 2 Control Sample Results Across Participating Laboratories

Laboratory	Sample	ALK fusion	ROS1 fusion	RET fusion	NTRK1 fusion	ALK 3′/5′ imbalance	ROS1 3′/5′ imbalance	RET 3′/5′ imbalance	NTRK 3′/5′ imbalance
INSERM	Cell Line RNA cocktail^a	Detected	Detected	Detected	n/a	0.0044	0.3031	0.0603	n/a
Kinki	Cell Line RNA cocktail^a	Detected	Detected	Detected	n/a	0.0405	0.4648	0.0434	n/a
ARUP	Cell Line RNA cocktail^a	Detected	Detected	Detected	n/a	0.0172	0.6141	0.0421	n/a
ARC-Net	Cell Line RNA cocktail^a	Detected	Detected	Detected	n/a	0.0086	0.3458	0.0353	n/a
Viollier	Cell Line RNA cocktail^a	Detected	Detected	Detected	n/a	0.043	0.3398	0.046	n/a
Radboudumc	Cell Line RNA cocktail^a	Detected	Detected	Detected	n/a	0.0414	0.2502	0.0856	n/a
CROM	Cell Line RNA cocktail^a	Detected	Detected	Detected	n/a	0.012	0.054	0.0813	n/a
IPATIMUP	Cell Line RNA cocktail^a	Detected	Detected	Detected	n/a	0.0238	0.2877	0.0539	n/a
Warwick	Cell Line RNA cocktail^a	Detected	Detected	Detected	n/a	0.0064	0.3	0.055	n/a
Queen’s	Cell Line RNA cocktail^a	Detected	Detected	Detected	n/a	0.0123	0.4158	0.0478	n/a
ARUP	FFPE cell block H2228	Detected	n/a	n/a	n/a	0.0312	n/a	n/a	n/a
ARUP	FFPE cell block HCC78	n/a	Detected	n/a	n/a	n/a	3.8313	n/a	n/a
ARUP	FFPE cell block LC-2/ad	n/a	n/a	Detected	n/a	n/a	n/a	0.2521	n/a
Kinki	Fresh cells H3122	Detected	n/a	n/a	n/a	0.3165	n/a	n/a	n/a
ARUP	FFPE cell block KM-12	n/a	n/a	n/a	Not covered	n/a	n/a	n/a	0.076

ISSN: 1471-2407