Fig. 4From: Deficiency in hormone-sensitive lipase accelerates the development of pancreatic cancer in conditional KrasG12D micea Representative histology (H.E. staining) of the pancreas of Hsl−/−, KC;Hsl+/+, KC;Hsl+/−, and KC;Hsl−/− mice. Scale bar represents 100 μm. No pancreatic neoplastic lesions were detected in Hsl−/− mice. Analysis of PDAC incidence at 20 weeks (sacrifice) in KC;Hsl+/+, KC;Hsl+/−, and KC;Hsl−/− mice (table right). b Ki67 immunohistochemistry of the pancreas of KC;Hsl+/+ and KC;Hsl−/− mice. Scale bar represents 100 μm. Quantification of Ki67 immunoreactivity in epithelial (ductal, indicated by solid arrows) and stromal cells (indicated by hollow arrows). N = 6, *: p < 0.01. c Immunofluorescence staining of the pancreas of KC;Hsl+/+ and KC;Hsl−/− mice (two representative mice in each group). Scale bar represents 100 μm. Red and green staining denote F4/80 (macrophage marker) and TNF-α (as a marker of tissue inflammation), respectively. Semi-quantitative analysis of TNF-α expressing macrophages (orange staining) in KC;Hsl+/+ and KC;Hsl−/− mice (table below)Back to article page