Fig. 3

a Representative HSL immunohistochemistry of the pancreas in wildtype, KC;Hsl^+/+, and KC;Hsl^−/− mice showing HSL positivity in pancreatic islets and PanIN cells. Scale bar represents 100 μm. b HSL western blot of total pancreatic lysates in KC;Hsl^+/+ and KC;Hsl^−/− mice (GAPDH as loading control). PCR analysis of Hsl transcripts in differentiated 3 T3-L1 (adipocytes), murine PanIN (mPanIN) and murine PDAC cells (both isolated from KC mice), murine pancreas and liver (lower). c Quantification of PL, TG, and FFA content in the pancreas of KC;Hsl^+/+ and KC;Hsl^−/− mice (n = 5). *: p < 0.01