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Fig. 4 | BMC Cancer

Fig. 4

From: A suppressive role of guanine nucleotide-binding protein subunit beta-4 inhibited by DNA methylation in the growth of anti-estrogen resistant breast cancer cells

Fig. 4

The ectopic expression of GNB4 causes alterations in cell cycle and apoptosis. a and b, 182R-6 and TAMR-1 cells stably expressing GNB4 or GFP grown to 90% confluency were subjected to cell cycle analysis using a GFP-Certified Nuclear-ID Red Cell Cycle Analysis Kit according to the manufacturer’s instructions. c and d, 182R-6 and TAMR-1 cells stably expressing GNB4 or GFP grown to 90% confluency were subjected to apoptosis analysis using an Annexin V-Cy3 Apoptosis Kit Plus according to the manufacturer’s instructions. Asterisk indicates p < 0.05

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