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Fig. 5 | BMC Cancer

Fig. 5

From: Differential expression of Cosmc, T-synthase and mucins in Tn-positive colorectal cancers

Fig. 5

Expression of Cosmc and T-synthase in human CRCs. a, mRNA levels of Cosmc and T-synthase. Relative mRNA levels were calculated using the 2-Ī”Ī”Ct method, with human Ī²-Actin mRNA as internal control. Paired samples are connected by a solid line. Cosmc and T-synthase mRNA levels were significantly higher in the tumors (pā€‰<Ā 0.05 andā€‰<ā€‰0.01, respectively). b, expression of Cosmc and T-synthase proteins in human CRCs. Case numbers are listed on top. Molecular weight is indicated at left, and protein names at right. Cosmc and T-synthase protein levels were elevated in the majority of tumors (T) compared to the matched normal tissues (N). c, relative expression levels of Cosmc and T-synthase proteins in CRCs. Cosmc and T-synthase protein levels detected by WB (b) were quantified using ImageJ and normalized to the Ī²-Actin protein. Paired samples are connected by a solid line. Cosmc and T-synthase protein levels were elevated in the tumors (pā€‰=ā€‰0.0007 andā€‰=ā€‰0.0015, respectively). d, correlation of Cosmc and T-synthase protein levels in human CRCs. Twenty-four tumors were plotted for their Cosmc/Actin and T-synthase/Actin ratios. Correlation was determined using Pearson Correlation Coefficient, and rā€‰=ā€‰0.8887 (pā€‰<ā€‰0.0001). e, T-synthase enzyme activities in matched human CRC samples. Fourteen of 24 cases had increased T-synthase activities in the tumor (>ā€‰2 fold change) compared to the matched normal control. White and black bars represent normal and tumor tissues, respectively. Case numbers are listed at the bottom of each panel. Error bars represent the standard error of the mean (SEM) calculated from triplicate

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