Fig. 2
From: Differential expression of Cosmc, T-synthase and mucins in Tn-positive colorectal cancers
Revertible expression of the Tn antigen in SW480 cells. a, flow cytometry analyses of SW480 Tn-positive subpopulation. SW480 parental cells were stained with anti-Tn antibody (BaGs6) and separated into Tn(ā) and Tn(+) subpopulations. The Tn(+) subpopulation was sorted into 96-well plates to form single clones. After expansion, these clones were analyzed for Tn expression. While single Tn(+) cells were isolated, their derived clones showed either negative (Clone#33) or heterogeneous (Clone#3, 15 and 34) Tn expression. In parental cells, histograms of isotype control and the BaGs6 staining are shown as red and green lines, respectively. b, Summary of Tn-positivity in single cell derived clones. Among a total of 52 clones examined, 19 are negative for the Tn antigen, 33 have a portion of Tn(+) cells, and none expresses the Tn antigen homogenously. c, expression of the Tn antigen, T-synthase, Cosmc, and Ī±-tubulin in SW480 cells. SW480-Tn(ā) and -Tn(+) cells were separated by FACS and extracted for total proteins. SW480-Tn(+) cells showed additional band representing the Tn-bearing protein(s) (arrows). There were comparable levels of T-synthase and Cosmc proteins in SW480-Tn(+), Tn(ā) and parental cells. Names of cell populations are listed on top. Protein standards are labeled at left, and antibodies at right. d, T-synthase activities in SW480 subpopulations. Both Tn(ā) and Tn(+) cells have comparable T-synthase activities with the parental cells. Activity values were determined in triplicates, error bars represent the standard error of the mean (SEM)