Average score per sample on 2 points
|
1.5
|
1.3
|
1.7
|
1.5
|
2.0
|
1.7
|
1.7
|
1.9
|
1.6
|
1.9
|
1.7
|
Score criteria
|
# obtained scores (%)
|
Correct (2 points)
|
11 (35.5%)
|
7 (22.6%)
|
12 (38.7%)
|
8 (25.8%)
|
23 (74.2%)
|
5 (16.1%)
|
4 (12.9%)
|
29 (93.5%)
|
18 (58.1%)
|
30 (96.8%)
|
147 (47.4%)
|
Correct but unspecified (2 points)
|
5 (16.1%)
|
3 (9.7%)
|
4 (12.9%)
|
2 (6.5%)
|
0 (0.0%)
|
16 (51.6%)
|
16 (51.6%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
46 (14.8%)
|
False-negative ≤ LOD (2 points)a
|
0 (0.0%)
|
5 (16.1%)
|
0 (0.0%)
|
4 (12.9%)
|
0 (0.0%)
|
0 (0.0%)
|
2 (6.5%)
|
0 (0.0%)
|
6 (19.4%)
|
0 (0.0%)
|
17 (5.5%)
|
False-negative but variant not tested for (2 points)a
|
0 (0.0%)
|
0 (0.0%)
|
3 (9.7%)
|
3 (9.7%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
6 (1.9%)
|
False-negative >/unknown LOD (0 points)a
|
1 (3.2%)
|
5 (16.1%)
|
3 (9.7%)
|
4 (12.9%)
|
0 (0.0%)
|
0 (0.0%)
|
1 (3.2%)
|
0 (0.0%)
|
3 (9.7%)
|
0 (0.0%)
|
17 (5.5%)
|
False-positive (0 points)a
|
2 (6.5%)
|
2 (6.5%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
1 (3.2%)
|
0 (0.0%)
|
0 (0.0%)
|
1 (3.2%)
|
6 (1.9%)
|
Incorrect mutation detected with therapeutic implications (0 points)a
|
0 (0.0%)
|
0 (0.0%)
|
1 (3.2%)
|
1 (3.2%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
2 (0.6%)
|
Incorrect mutation detected without therapeutic implications (2 points)
|
3 (9.7%)
|
1 (3.2%)
|
0 (0.0%)
|
1 (3.2%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
5 (1.6%)
|
One variant missed in double mutation sample (0 points)a
|
N/A
|
N/A
|
N/A
|
N/A
|
N/A
|
N/A
|
N/A
|
2 (6.5%)
|
4 (12.9%)
|
N/A
|
6 (1.9%)
|
Mutation described incorrectly (1 point)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
10 (32.3%)
|
6 (19.4%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
16 (5.2%)
|
Technical failure (not scored)
|
1 (3.2%)
|
0 (0.0%)
|
0 (0.0%)°
|
0 (0.0%)°
|
0 (0.0%)
|
0 (0.0%)
|
1 (3.2%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
2 (0.6%)
|
Not tested (not scored)
|
8 (25.8%)
|
8 (25.8%)
|
8 (25.8%)
|
8 (25.8%)
|
8 (25.8%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
0 (0.0%)
|
40 (12.9%)
|
Total scored (n = 31)
|
22 (71.0%)
|
23 (74.2%)
|
23 (74.2%)
|
23 (74.2%)
|
23 (74.2%)
|
31 (100.0%)
|
30 (96.8%)
|
31 (100.0%)
|
31 (100.0%)
|
31 (100.0%)
|
268 (86.5%)
|
Error rate
|
# obtained scores (%)
|
Total with implication on therapy decisiona
|
3 (13.6%)
|
12 (52.2%)
|
7 (30.4%)
|
12 (52.2%)
|
0 (0.0%)
|
0 (0.0%)
|
4 (13.3%)
|
2 (6.5%)
|
13 (41.9%)
|
1 (3.2%)
|
54 (20.1%)
|
Gene not tested/No method info given
|
0
|
0
|
3
|
3
|
0
|
0
|
0
|
0
|
0
|
0
|
6
|
NGS
|
2
|
7
|
3
|
7
|
0
|
0
|
1
|
1
|
7
|
1
|
29
|
Commercial Kit
|
0
|
3
|
1
|
1
|
0
|
0
|
0
|
0
|
1
|
0
|
6
|
LDT
|
1
|
1
|
0
|
0
|
0
|
0
|
1
|
0
|
1
|
0
|
4
|
BEAMing
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
ddPCR
|
0
|
1
|
0
|
1
|
0
|
0
|
2
|
1
|
4
|
0
|
9
|