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Fig. 3 | BMC Cancer

Fig. 3

From: Daphnane diterpenes inhibit the metastatic potential of B16F10 murine melanoma cells in vitro and in vivo

Fig. 3

In vitro evaluation of the effect of daphnane diterpenes on B16F10 cells metastasis. a Cell proliferation evaluated using the MTT assay; b Viability of B16F10 cells treated with 1/000 v/v T. hirsuta extract (TH) evaluated using flow cytometry (Guava ViaCount assay); c Scratched cell monolayer images for the wound healing assay results (cell migration assay); d Wound healing assay results; e Cell invasion results obtained using the matrigel invasion assay; f Cell adhesion assay results evaluated by performing the MTT assay on B16 cells cultured in fibronectin-coated 96-well plates. Results represent the mean ± SD of triplicate samples. **Statistically significant (P ≤ 0.01) difference between treated cells and control

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