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Fig. 2 | BMC Cancer

Fig. 2

From: Daphnane diterpenes inhibit the metastatic potential of B16F10 murine melanoma cells in vitro and in vivo

Fig. 2

Effect of daphnane diterpenes on the expression of MMP2 and CD44 in vivo. The expression of (a) CD44 (b) and metalloproteinases MMP9 and MMP2 in mice injected without (−) B16F10 or with (+) B16F10, and treated with dacarbazine (DTIC) or 1/1000 v/v T. hirsuta extract (TH). Total proteins were then extracted and resolved by SDS-PAGE; the resolved proteins were then blotted onto a PVDF membrane. CD44, MMP2, and MMP9 were detected by immunoblotting with an anti-CD44, anti-MMP2, or anti-MMP9, polyclonal antibodies. The signals were visualized using LiCor Odyssey Infrared Imaging System after reaction with goat anti-mouse IRDye 680LT, or goat anti-rabbit IRDye 800CW (LI-COR). CD44, MMP2, and MMP9 levels were normalized to β-actin expression

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BMC Cancer

ISSN: 1471-2407

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