Fig. 5
CSC spheroids possessed higher tumorigenic capability: 12 week old C57L/J mice were injected with 2 × 106 control Hepa1–6 cells or Hepa1–6 spheroids (n = 6 mice/group) into left liver lobe. Tumors were allowed to grow for two weeks and mice were euthanized at the end of two weeks; a Representative liver tumors of control and spheroid group mice; b Representative H&E staining of control group and spheroid group, bright field images with 10× magnification (upper, bar = 200 μm) and 40× magnification (lower, bar = 50 μm); c Quantitative comparison of body weights, liver weights, tumor weights, and tumor volumes between control and spheroid group mice. *(p ≤ 0.05). NS (non-significant)