Fig. 4

Silencing β-catenin reverses spheroid phenotype of CSCs: a Hepa1–6; b HepG2 cells were cultured as control or spheroids for 3 days, transient transfection with scrambled siRNA control or siCTNNB1, followed by 48 to 72 h incubation. Upper panels show average quantification (n = 5 random fields/group) of spheroids (orange), adherent (blue), and single cells (green). Error bar is representing SD. n = 3 independent experiments, *** (p ≤ 0.005, control), ### (p ≤ 0.005, spheroids). Lower panel show visual representative images of corresponding experimental group