Fig. 4

miR17–92 cluster mediates the regulation between MYC and TXNIP. a JQ1 treatment repressed the expression of miR-17-92 rapidly. b The expressions of mature miRNAs from the miR-17-92 cluster were inhibited by JQ1 treatment. c MOLM-14 and KG1 cells were transfected with miRNA inhibitors, cultured for 24 h, and the expression level of TXNIP was examined. d 293 T cells were transfected with luciferase vectors carrying wild type (WT) of mutant (MUT) 3’UTR of TXNIP before treatment with JQ1 (2 μM), transfected with MYC siRNAs, MYC expressing vector, or miRNA inhibitors. Cells were cultured for 24 h under different treatments before being harvested for luciferase activity assay. The final luciferase activities were normalized to total protein concentration and measured with Bradford assay. e Proposed model of MYC-TXNIP regulatory cascade