Fig. 5

Knockout of BAG3 expression in HCT-116 cells. a A fluorescence microscope was used to confirm the transfection efficiency in HCT-116 cells after 72 h of lentiviral transfection. b DNA agarose gel electrophoresis was performed to confirm the knockout of the target gene. c Western blot analysis was used to examine the expression of BAG3 protein in knockout cells. Data represent mean ± S.D. from three independent experiments