Fig. 2

Differential AKT1 and AKT2 expression and activation across cell lines a-d Correlations of protein or phospho-protein levels of AKT1 and AKT2. The 211 cell lines were cultured in MD Anderson RPPA core. RPPA data for 211 cell lines were analyzed for correlations between AKT1 and AKT2 (a), pAKT1 Ser473 and pAKT2 Ser474 (b), AKT1 and pAKT1 Ser473 (c), and AKT2 and pAKT2 Ser474 (d). Spearman rank correlation coefficient and Pearson correlation coefficient are presented. Arrows in (b) designate HEC151 and HEC50 cells that were further analyzed. e Protein and phospho-protein levels of AKT1 and AKT2 in HEC151 and HEC50 cells. Two endometrial adenocarcinoma cell lines (HEC151 and HEC50) and other cell lines including HCT116 DKO, parental HCT116, and HOC8 were cultured in complete medium. Lysates (30 μg/lane) were resolved in 10% SDS PAGE. Antibodies for each blot are listed to the left