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Fig. 1 | BMC Cancer

Fig. 1

From: CXCR4 and its ligand CXCL12 display opposite expression profiles in feline mammary metastatic disease, with the exception of HER2-overexpressing tumors

Fig. 1

Immunohistochemical and immunofluorescence analysis of CXCR4 and CXCL12 expression in normal mammary tissue, in primary tumors (PT) and in metastatic lesions reflects the pathology of FMC. Photomicrographs (a, b, e and f) represent immunohistochemical control samples of normal tissue stained for CXCR4 (a) and CXCL12 (e) and PT in the absence of CXCR4 (b) and CXCL12 (f) antibody incubation. Photomicrographs (c and d) show representative samples of PT (c) and regional metastasis (RM) (d) with CXCR4 positive staining in cell membrane and cytoplasm. Photomicrographs (g and h) illustrate representative samples of PT (g) and RM (h) with CXCL12 positive staining observed mainly in the cytoplasm. Photomicrograph (l) represents immunolabeling of PT cells in appropriate fluorescence filter sets, corresponding to the signals of DAPI (i), CXCR4-Alexa Fluor® 594 (j) and CXCL12-Alexa Fluor® 488 (k). All photomicrographs were taken in the high-powered magnification, × 400 and the scale bar represents 40 μm

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