Fig. 1

ASP1 is up-regulated in GBM. a Oncomine analysis of LASP1 expression in two independent GBM databases; (b) Kaplan-Meier survival analysis of 149 GBM patients from TCGA; patients were divided into a high group (n = 75) and low group (n = 74) according to LASP1 expression; (c) western blot and real-time PCR analysis were performed to detect LASP1 expression in 4 GBM cell lines; (d) real-time PCR analysis of LASP1 mRNA expression in paired human GBM tissues (n = 34) and their adjacent normal tissues (n = 34); (e) western blot analysis of LASP1 expression in 4 paired samples of GBM tissues versus adjacent normal tissues. The immunosignal was quantified by greyscale scanning software Quantity One, and the relative protein abundance was normalized by GAPDH. ^* P < 0.05, ^** P < 0.01, ^***p < 0.001