Table 1 Antibodies used in this study including clone, dilution, and pretreatment
Marker | Company, clone | Dilution | Pretreatment | Visualization |
|---|---|---|---|---|
Brk | Biosource | 1:200 | CC1mild | UV/DAB |
CdC42 | Santa Cruz | 1:50 | MW6,0 | ENV/DAB |
Connexin 43/GJA1 | Novusbio | 1:500 | CC1mild | UV/DAB |
CXCR1 | Abcam | 1:200 | MW9,0 | ENV/DAB |
CXCR2 | Abcam | 1:200 | MW9,0 | ENV/DAB |
CXCR4 | Abcam 124824 | 1:500 | retrievalDAKO WB | HRB-DAB |
Ecadherin | Dako | Ready to use | high ph | Omnis Flex |
Ncadherin (5D5) | Abcam | 1:500 | CC1mild | UV/DAB |
ERK1/2 p44/42MAPK | Cell signaling | 1:100 | MW Tris | ENV/DAB |
Focal Adhesion kinase FAK | Abcam | 1:1000 | MW6,0 | ENV/DAB |
Fascin 1 | Chem | 1:500 | MW6,0 | CM/AEC |
Integrin-linked ILK phosphoS246 | Abcam | 1:100 | CC1mild | UV/DAB |
Mad | Novusbio | 1:100 | CC1mild | UV/DAB |
PI3K p110α | Cell signaling | 1:50 | MW9,0 | CM/AEC |
PLCγ | Abcam | 1:100 | MW9,0 | ENV/DAB |
Rab40B | Proteintech | 1:200 | MW6,0 | ENV/DAB |
Rack1 | Spring | 1:100 | CC1mild | UV/DAB |
RhoA | Abcam | 1:200 | MW6,0 | ENV/DAB |
SARI/BATF2 (MGC20410) | Novusbio | 1:200 | CC1mild | UV/DAB |
SAX | Abcam | 1:200 | CC1mild | UV/DAB |
Slug | Novusbio | 1:100 | CC1mild | UV/DAB |
SMARCA4/BRG1 | Abcam | 1:200 | CC1mild | UV/DAB |
Snail | Santa Cruz | 1:50 | MW6,0 | ENV/AEC |
pSRC phosphoS75 | Abcam | 1:100 | CC1mild | UV/DAB |
TGF1β | Santa Cruz | 1:50 | CC1mild | UV/DAB |
Tks5 | Novusbio | 1:75 | MW6,0 | ENV/DAB |
Twist(10E4E6) | Novusbio | 1:50 | MW6,0 | ENV/DAB |
Vim | Dako | Ready to use | low ph | Omnis Flex |
YAP phospho127 | Abcam | 1:300 | CC1mild | UV/DAB |
ZEB1/ARBE6 | Abcam | 1:200 | CC1mild | UV/DAB |
ZFPL1 | Abcam | 1:200 | CC1mild | UV/DAB |