Fig. 2

Immunohistochemistry in an area of vascular invasion; a) Twist, one arrow pointing to the carcinoma complexes (left), the other to the vascular wall (top), b) ZEB1, tumor cell nuclei are all negative; note the positively stained stroma cells, c) E-Cadherin, the tumour bulks are stained within the vascular wall, d) N-Cadherin, positively stained acini in the invasion front, e) vimentin, compare light staining of tumour cells and strong staining of the stroma cells, vimentin positive (single arrow) and negative (double arrow) tumour cells, f) cytokeratin, g) Fascin; magnification bars 50 μm (a-c, e-g), and 20 μm (d), respectively