Fig. 6

Expression of IFIT3 gene in human breast cell lines: a MCF7, MDA-MB-231, MCF10A, and primary cell lines b BrCCh1, BN1, BC5 after 24 h transfection with an analog of U25 C/D box in the presence Lipofectamine 3000. Quantitative RT-PCR values were normalized to level of GAPDH, HPRT and RNU6 RNAs. Results are plotted relative to cells incubated with Lipofectamine 3000 for sample transfected with RNA and to non-treated cells for samples under INFα exposure. Data shown represent the mean ± SD from two independent experiments. The difference between the groups was statistically significant at p < 0.05 (*) and at p < 0.01 (**)