Fig. 5

IL8 inhibition phenocopy telomerase inhibition. a HCT116 and OVCAR5 cell lines stably expressing a non-specific (NS) shRNA or IL8 shRNAs. Knockdown is determined by measuring IL8 mRNA levels and plotting with respect to the control cell expressing nonspecific shRNA. b Cell viability of the cells expressing either nonspecific or IL8 shRNA was measured by trypan blue exclusion assay. Cell viability relative to control cell expressing nonspecific shRNA is plotted. HCT116 cells were either treated with mismatch oligonucleotide or imetelstat for 2 weeks and were then transfected to overexpress IL8-GFP tagged cDNA. c Western blot for GFP tag was performed to check IL8 overexpression in the cells. d Cell viability was measured by trypan blue exclusion assay and plotted with respect to control mismatch oligonucleotide treated cells. e Telomerase activities was measured by TRAP assay and plotted with respect to control mismatch oligonucleotide treated cells. Error bar shows Standard Error Mean (SEM). (**, p < 0.001 and *, p < 0.01)