Fig. 2

Effects of 3AOA on the expression of VEGF family proteins in SCCVII cells treated with CoCl₂ . a, cDNAs were generated from total RNAs treated with DNase I, and PCR reaction was performed with specific primers of VEGF-A, -B, -C, −D and GAPDH. b, PCR products from three independent experiments (a) were quantified and represented as a bar diagram. The levels of the VEGF-A, -B, -C, and -D transcripts in the control (3AOA- and CoCl₂-untreated cells) were estimated as 100%. c, The protein level of VEGF-A in the intracellular fraction was determined using western blot with anti-VEGF-A antibody. d, The secreted VEGF-A protein level in a conditioned medium was determined using an ELISA assay. The amounts of VEGF-A obtained in three independent experiments were quantified and represented as a bar diagram. The level of VEGF-A in the conditioned medium of the control was estimated as 100%. Data are presented as a mean ± S.D. of three independent experiments (^*p < 0.05, ^**p < 0.01, ^***p < 0.001)