Fig. 1

Effects of 3AOA on tumor growth and lymphangiogenesis in an oral cancer sentinel lymph node animal model. a, Tumor growth and metastasis to sentinel lymph node b, Tumor volume c, Image of sentinel lymph nodes d, Sentinel lymph node volume e-f, Lymphatic vessels in tumor and sentinel lymph node sections. Tumor growth of each group was confirmed by hematoxylin and eosin staining of tumor(tongue) sections. And metastasis to sentinel lymph node was confirmed using hematoxylin and eosin staining, and immunohistochemical analysis with anti-cytokeratin antibody of sentinel lymph node sections. Tumor sections were digitized and microscopic images were captured under a 100× objective magnification. Scale bar = 200 μm. Sentinel lymph node sections were digitized and microscopic images were captured under a 200× objective magnification. Scale bar = 200 μm. Lymphatic vessels in tumor and sentinel lymph node sections were determined using the immunohistochemical analysis with anti-LYVE-1. All sections were digitized and microscopic images were captured under a 200× objective magnification. Scale bar = 200 μm. Immunohistochemical intensity values of LYVE-1 from captured images were analyzed by the Image J program and represented as a bar diagrams. Data are presented as a mean ± S.D. (^*p < 0.05, ^**p < 0.01, ^***p < 0.001). T = tumor; SLN = Sentinel lymph node