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Table 2 Details of primers used in the present study

From: Aberrant Promoter Methylation of YAP Gene and its Subsequent Downregulation in Indian Breast Cancer Patients

Primer Primer Sequence PCR product size (base pair) Annealing Temperatu-re (°C)
Mutation primers
YAP1 exon 1 (serine 61) F 5’-AGGCAGAAGCCATGGATC-3’ 338 56.6
R 5’-GGTTACCTGTCGGGAGTG-3’
YAP1 exon 2 (serine 109 and 127) F 5’-GGCTGCAATTAAGCGCTGAC-3’ 292 61.5
R 5’-TGCTGGCAGAGGTACATCATC-3’
YAP1 exon 2 (serine 164) F 5’-CGAGCTCATTCCTCTCCAGC-3’ 236 55.5
R 5’-AGATAACTGTCTCCCACC-3’
YAP1 exon 8 (serine 397) F 5’-TTCAGACATTGCAGGACAGG-3’ 248 58.8
R 5’-CCTGTATCCATCTCATCCACAC-3’
YAP1 exon 9 (lysine 494) F 5’-CTCTGTGTGTTTCCACTAGG-3’ 317 57.5
R 5’-CCGGTGCATGTGTCTCCTTAG-3’
Methylation primers
YAP1 methylation F 5’-AGTTCGTATAGGCGTTTCGTTC-3’ 187 57.9
F 5’-CTTAACTACAAAAAATTCTTCCGCT-3’
YAP1 unmethylation F 5’-AAGTTTGTATAGGTGTTTTGTTTGG-3’ 188 57.9
F 5’-CTTAACTACAAAAAATTCTTCCACT-3’
Expression primers
YAP1 F 5’-AAGCTGCCCGACTCCTTCTTCAAG-3’ 161 64.1
R 5’-GTCAGTGTCCCAGGAGAAACA-3’
GAPDH F 5’-CACTGCCACCCAGAAGACTG-3’ 150 61
R 5’-ATGCCAGTGAGCTTCCCGTT-3’