Table 2 Details of primers used in the present study
Primer | Primer Sequence | PCR product size (base pair) | Annealing Temperatu-re (°C) |
Mutation primers | |||
YAP1 exon 1 (serine 61) | F 5’-AGGCAGAAGCCATGGATC-3’ | 338 | 56.6 |
R 5’-GGTTACCTGTCGGGAGTG-3’ | |||
YAP1 exon 2 (serine 109 and 127) | F 5’-GGCTGCAATTAAGCGCTGAC-3’ | 292 | 61.5 |
R 5’-TGCTGGCAGAGGTACATCATC-3’ | |||
YAP1 exon 2 (serine 164) | F 5’-CGAGCTCATTCCTCTCCAGC-3’ | 236 | 55.5 |
R 5’-AGATAACTGTCTCCCACC-3’ | |||
YAP1 exon 8 (serine 397) | F 5’-TTCAGACATTGCAGGACAGG-3’ | 248 | 58.8 |
R 5’-CCTGTATCCATCTCATCCACAC-3’ | |||
YAP1 exon 9 (lysine 494) | F 5’-CTCTGTGTGTTTCCACTAGG-3’ | 317 | 57.5 |
R 5’-CCGGTGCATGTGTCTCCTTAG-3’ | |||
Methylation primers | |||
YAP1 methylation | F 5’-AGTTCGTATAGGCGTTTCGTTC-3’ | 187 | 57.9 |
F 5’-CTTAACTACAAAAAATTCTTCCGCT-3’ | |||
YAP1 unmethylation | F 5’-AAGTTTGTATAGGTGTTTTGTTTGG-3’ | 188 | 57.9 |
F 5’-CTTAACTACAAAAAATTCTTCCACT-3’ | |||
Expression primers | |||
YAP1 | F 5’-AAGCTGCCCGACTCCTTCTTCAAG-3’ | 161 | 64.1 |
R 5’-GTCAGTGTCCCAGGAGAAACA-3’ | |||
GAPDH | F 5’-CACTGCCACCCAGAAGACTG-3’ | 150 | 61 |
R 5’-ATGCCAGTGAGCTTCCCGTT-3’ | |||