Fig. 5
From: MAFb protein confers intrinsic resistance to proteasome inhibitors in multiple myeloma
Knockdown of MAFb restores sensitivity of myeloma to PIs. SACHI cells were infected with lentiviral expression system containing shRNA specific to MAFb gene (shMAFb) or shRNA containing scramble sequences (shCon) for 48 h. MAFb mRNA was measured by RT-qPCR analysis (a), MAFb protein in whole lysis buffer was analyzed by immunoblotting analysis (b) and in both cytoplasmic and in nucleus by immunofluorescent staining analysis (c) as described in Fig. 5d. MAFb target genes in shMAFb and shCon cells were analyzed by qRT-PCR analysis (d). The cells were treated with indicated concentrations of Bzb (e) or CFZ (f) for 48 h and cell survival was measured by MTT assay. Results are presented as mean ± SE (n = 4). Data are representative of 3 separate experiments. *P < 0 .01 versus control. **P < 0 .001 versus control