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Fig. 7 | BMC Cancer

Fig. 7

From: MASTL inhibition promotes mitotic catastrophe through PP2A activation to inhibit cancer growth and radioresistance in breast cancer cells

Fig. 7

MASTL depletion sensitizes radioresistant breast cancer stem cells. CD44high/CD24low MCF7 cells were transfected with either 5 nmol/l control siRNA or MASTL.5 siRNA. The cells were irradiated with 0, 3, or 4 Gy for 42 h. a, b The clonogenic assay results. a Representative images of the cells treated with the indicated conditions. b The number of colonies was measured. c The cells were analyzed by immunoblotting with the indicated antibodies. d, e The sphere forming assay was performed. Scale bars = 100 μm. d Representative images of sphere forming assay in Ctrl MCF7 and CD44high/CD24low MCF7 cells. e The sphere forming capacity was measured from the sphere diameter (μm) (> 110 sphere colonies per data point). The data represent typical results and are presented as the mean ± standard deviation of three independent experiments; **P < 0.01 and *P < 0.05

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