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Fig. 6 | BMC Cancer

Fig. 6

From: MASTL inhibition promotes mitotic catastrophe through PP2A activation to inhibit cancer growth and radioresistance in breast cancer cells

Fig. 6

MASTL depletion increases the radiosensitivity of breast cancer cells. MCF7 cells were transfected with either 5 nmol/l control siRNA or MASTL.5 siRNA. Cells were treated 0, 3 or 4 Gy irradiation for 42 h. a, b Cell proliferation was determined by FACS analysis and then analyzed by immunoblotted with the indicated antibodies. c The results of the clonogenic assay. Representative images of the cells treated the indicated conditions (left panel). The number of colonies was measured (right panel). d The sphere forming assay was performed (> 110 sphere colonies per data point). Scale bar = 100 μm. Representative images of sphere forming assay. e PP2A-Aα/β proteins were immunoprecipitated using an anti-PP2A-Aα/β antibody and analyzed for PP2A activity. The data represent typical results and are presented as the mean ± standard deviation of three independent experiments; **P < 0.01 and *P < 0.05

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