Fig. 3

MASTL depletion reduces the oncogenic properties of breast cancer cells. MCF7 and T47D cells were transfected with either control siRNA, MASTL.4 siRNA, or MASTL.5 siRNA for 48 h. Colony formation was determined by using a soft agar assay by a representative images and b, c the quantification of colony formation of MCF7 and T47D cells. d, e, f Mammosphere formation was determined by using sphere formation assay by d representative images and e, f quantification of tumor sphere formation in MCF7 and T47D cells. Scale bars = 100 μm. The data represent typical results and are presented as the mean ± standard deviation of three independent experiments; **P < 0.01 and *P < 0.05