Fig. 2From: MASTL inhibition promotes mitotic catastrophe through PP2A activation to inhibit cancer growth and radioresistance in breast cancer cellsMASTL depletion induces cell death in breast cancer cells. The cells were transfected with either the control (Ctrl) siRNA or MASTL siRNAs, as indicated, for 48 h. a Representative light microscopy images of control (Ctrl) or MASTL.1 siRNA-depleted cells. Scale bar = 100 μm. b The cells were analyzed by immunoblotting or c immunofluorescence analysis with the indicated antibodies. Scale bars = 10 μm. d MCF7, T47D, HCC1937, and SKBR3 cells treated with either control (Ctrl) siRNA or MASTL.5 siRNA were analyzed by immunoblotting with the indicated antibodies. e The trypan blue exclusion assay was used to assess the effect of the cells treated with either control (Ctrl) siRNA, MASTL.4 siRNA, or MASTL.5 siRNA. f MCF7 cells were transfected with either control (Ctrl) siRNA, MASTL.5 siRNA, or both MASTL.5 siRNA and mouse MASTL vector for 48 h and then analyzed by immunoblotting for the indicated antibodies. The data represent typical results and are presented as the mean ± standard deviation of three independent experiments; **P < 0.01 and *P < 0.05Back to article page