Fig. 2

Immunolocalization of cell HSPGs. a-d, Histological localization of syndecan-1 expression. a, Normal mucosa showing intense staining associated with the cell membrane. Plasma cells in lamina propria are also stained positively. b, Non-metastatic tumor displaying decreased immunoreactivity and weak staining in ECM (arrows). Plasma cells in lamina propria are also positively stained; magnification 40X. c, Metastatic tumor and (d), Transition area between normal colon mucosa and tumor; arrows indicate the loss of staining in the tumor region; magnification 20X and 10X respectively. e-f Immunolocalization of glypican-4 expression in normal mucosa (e) and metastatic tumor (f); In the healthy tissue weak immunomarking is observed in crypts and matrix which is stronger in the tumor tissue, as it also is in the fibrous tissue (arrow); magnification 40X. (g-h), Immunolocalization of mast cells using CD117 antibody. Localization of mast cells in normal colon mucosa (g) and tumor (h). Arrows indicate the staining of mast cells in the normal mucosa. The asterisks show the tumor area, where there is no detectable staining. Magnification 40X