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Fig. 1 | BMC Cancer

Fig. 1

From: Nm23-H1 is involved in the repair of ionizing radiation-induced DNA double-strand breaks in the A549 lung cancer cell line

Fig. 1

The expression and location of Nm23-H1 in A549-shNm23-H1 cell line and A549-nNm23-H1 cell line. A549-shNm23-H1 cell line was a Dox-regulated vector system of conditional suppressing the expression of Nm23-H1, and the Nm23-H1 expression was suppressed only when doxycycline was added. A549-nNm23-H1 cell line was constructed for over expressed Nm23-H1 with nuclear located sequence (NLS) which can introduce Nm23-H1 into nucleus. a The expression of Nm23-H1 in A549-shNm23-H1 cell line detected by Western blot. b The expression and location of Nm23-H1 in A549-nNm23-H1 cell line detected by Western blot NLS-Nm23-H1 is the constructed protein(18 kDa) and Nm23-H1 is the endogenous protein (17 kDa). 1 A549; 2 A549-vector; 3 A549-nNm23-H1. c The expression and location of Nm23-H1 detected by confocal microscopy in A549-nNm23-H1 cell line. The Nm23-H1 protein is stained with green fluorescent and the nucleus is stained with blue fluorescent . 1 A549; 2 A549-vector; 3 A549-nNm23-H1

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BMC Cancer

ISSN: 1471-2407

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