Skip to main content
Fig. 4 | BMC Cancer

Fig. 4

From: Differential expression of ABCB5 in BRAF inhibitor-resistant melanoma cell lines

Fig. 4

Knockdown of ABCB5 did not re-sensitize A2058 PLXr, SK-MEL-28 PLXr, and A375 cells to PLX treatment. A2058 PLXr cells (a), SK-MEL-28 PLXr cells (b), and A375 cells (c) were seeded in 24-well plates. Cells were transfected with either ABCB5 control siRNA or ABCB5 siRNA the next day. After 6 h of transfection, PLX was added to each well as designated. Cells that were not transfected were used as another group of control. Cell viability assay by MTT was performed after 72 h of PLX treatment. Results are expressed as the percent viable cells and values represent the means ± SD (bars) of three independent experiments. d A375 cells, PLX-resistant A2058 PLXr and SK-MEL-28 PLXr cells were seeded in 12-well plates. Cells were transfected with either ABCB5 control siRNA or ABCB5 siRNA the next day. After 12 h of transfection, total RNA was isolated. Real-time PCR was performed as described in Fig.2a (ctrl: control). e ABCB5 knockdown by ABCB5 siRNA was shown by Western blot in A2058PLXr cells. ABCB5/actin showed the ratio of the scanned optical density of the ABCB5 protein band

Back to article page