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Fig. 2 | BMC Cancer

Fig. 2

From: Differential expression of ABCB5 in BRAF inhibitor-resistant melanoma cell lines

Fig. 2

Differential expression of ABCB5, ERK, Akt, p-ERK, and p-AKT in wild-type BRAF cell, PLX-resistant melanoma cells and their parent cells by real-time PCR (a) and Western blot analysis (b). a Total RNA from melanoma cells and PLX-resistant melanoma cells were isolated. Real-time PCR was performed as described in Materials and Methods. B2M was used as an internal control. The bar graphs are expressed as fold changes of PLX resistant cell lines relative to each of their parent cell lines adjusted for B2M. Each of the experiments is a representation of two independent experiments performed in triplicate (** p < 0.01, n = 3). b Western blot analysis of ABCB5, ERK, Akt, p-ERK, p-Akt expression in wild-type BRAF cells, PLX-resistant cell lines, and their parent cell lines are shown. Anti-actin was used to show the equal loading and transfer in each lane. Targeted protein/actin showed the ratio of the scanned optical density of each protein band. ABCB5 protein level increased in A2058 PLXr and SK-MEL-28 PLXr cells versus A2058 and SK-MEL-28 cells. However, ABCB5 protein level was decreased in A375 PLXr cells versus A375 cells

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