Fig. 3

Growth regulatory role of TET1 in SI-NET cells. a Colony formation assay in the adhesive SI-NET cell line CNDT2.5. Cells transfected with a TET1 expression vector or empty vector were selected for blasticidin resistance in 10 days and then counted. A representative result is shown and below quantification of triplicate is shown. b Apoptosis was analyzed by quantifying cytoplasmic histone-associated-DNA-fragments 72 h after transient transfection. Incubation in 0.1 μg/ml camptothecin was used as positive control. *, p < 0.05. c TET1 expression at the mRNA and protein level was increased after transfection of the TET1 expression vector