Fig. 6
From: ALDH1A inhibition sensitizes colon cancer cells to chemotherapy
Induced apoptosis and necrosis in cells transfected with specific siRNA measured by Annexin V assay. Concentrations of chemotherapeutics used in this assay were as follow: 5-FU: 5 μg/ml for HCT-116/eGFP, 20 μg/ml for HT-29/eGFP and LS-180/eGFP; RAL: 20 nM for HCT-116/eGFP and HT-29/eGFP, 50 nM for LS-180/eGFP; IRI: 20 μg/ml for HCT-116/eGFP and HT-29/eGFP, 25 μg/ml for LS-180/eGFP. a: HCT-116/eGFP, b: HT-29/eGFP, c: LS-180/eGFP