Fig. 2
Status of AMPK phosphorylation and marker of autophagy expression in Ishikawa endometrial cancer cells treated by liraglutide. a Ishikawa cells were treated with different concentrations of liraglutide for 96 h. The cells were harvested, and AMPK, p-AMPK, LC3-II and p62 expression was analyzed by Western blot. β-actin was used as an internal control. Representative Western blot results are shown. LC3-II is defined as the lower band in the panel. b Quantification of the p-AMPK/AMPK ratio. * denotes means significantly different from control (p <  0.05; ANOVA). c Quantification of the LC3-II/β-actin ratio. * denotes means significantly different from control (p <  0.05; ANOVA). d Quantification of the p62/β-actin ratio. * denotes means significantly different from control (p <  0.05; ANOVA)