Fig. 1

MG132 increases expression of p-STAT1 and STAT1 in ESCC cell lines. Western blot analysis demonstrates that the dose-dependent and time-dependent elevation of STAT1 induced by MG132. a. EC1 and KYSE150 cell lines were treated in the presence of 0–10 μM MG132 for 24 h. Total cell lysates were then prepared for immunoblot detection of p-STAT1^Y701 and p-STAT1^S727, STAT1 and β-actin. b. ESCC cell lines were treated with 10 μM MG132 and cells were harvested for immunoblot analysis at different time intervals. c. STAT1 transfection into EC1 and KYSE150 cells resulted in a dramatic increase in the levels of p-STAT1 and STAT1. Similar results were observed in three independent experiments. (E.V.: Empty vector)