Fig. 1

HA Influence the proliferation, wound healing, migration and adhesion of endothelial cells. a Cell survivability assay (MTT) was performed for untreated and HA-treated (10μg/ml) ECs to examine the effect of HA on proliferation rate. Three wells for each condition were taken for different time points. Same number of cells were seeded in triplicates for each time point along with a set of untreated cells as controls b Images of scratch assay showing the influence of HA on ECs migration to recover the wound. Scale bar, 100 μm. c Percentage of scratch covered after HA treatment were quantified after 24 and 48 h d Graph showing the number of migrated cells through the porous transwell membrane in untreated control and HA treated (10μg/ml for 48 h) ECs. e Graph showing change of adhesion property of cells with HA treatment (10μg/ml for 48 h) after 2 and 4 h in comparison to equal number of untreated control ECs. *p < 0.05; **p < 0.01, ***p < 0.001