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Fig. 2 | BMC Cancer

Fig. 2

From: Knock-down of LRP/LR promotes apoptosis in early and late stage colorectal carcinoma cells via caspase activation

Fig. 2

The effect of siRNA-mediated knock-down of LRP on nuclear morphology of early (SW-480) and late (DLD-1) stage colorectal cancer cells. Confocal microscopy with Airyscan analysis was completed to investigate changes in nuclear morphology upon siRNA treatment. a and e) Non-transfected SW-480 and DLD-1 cells displayed large nuclei with healthy membrane integrity. b and f) SW-480 and DLD-1 cells transfected with negative control esiRNA-RLUC showed parallel characteristics to the non-transfected cells with no changes in nuclear morphology. c) SW-480 cells treated with positive control PCA revealed nuclei that underwent apoptotic body formation. d) SW-480 cells transfected with siRPSA #1 exhibited nuclear shrinkage and condensed nuclei, proposing induction of apoptosis. g) DLD-1 cells treated with PCA displayed weakened membrane integrity and condensed nuclei. h) DLD-1 cells transfected with siRPSA #1 showed formation of apoptotic bodies and weakened membrane integrity. Images were obtained at a 630X magnification and Airy scan analysis was applied to each image. Scale bars are indicative of 20 μm

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