Fig. 6

Estrogen facilitates binding of ERRβ on half EREs through ERα. a ChIP assay showing ERα binding to half EREs on ERRβ promoter in-vivo in the presence of estrogen in (i) MCF7 and (ii) T47D cells. b ERRβ binding in the upstream region of ERRβ promoter upon estrogen treatment in (i) MCF7 and (ii) T47D cells. c ER-ve breast cancer cells (MDA-MB 231) showing no binding of ERα on ERRβ promoter and used as a negative control. d Re-ChIP shows binding of ERα and ERRβ heterodimer complex on half ERE sites present on ERRβ promoter