Fig. 4

Abnormal Cell cycle process and cell apoptosis in human lung adenocarcinoma cell lines with GMDS knockdown. a-b Cell cycle distribution in A549 cells (a) and H1299 cells (b) infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA. Both cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA were cultured for 72 h. After propidium iodide (PI) staining, cell cycle distribution was analyzed with flow cytometry. The graph represents the mean ± SEM of cell proportion in the G1 phase, S phase and G2/M phase from three independent experiments (*, p < 0.05; **, p < 0.01). c-d Cell apoptosis in A549 cells (c) and H1299 cells (d) infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA. Both cells infected with lentivirus expressing either Scr-shRNA or GMDS-shRNA were cultured for 4 days and cell apoptosis was analyzed using ANNEXIN-V assay in combined with flow cytometry. Data shown are the mean ± SEM of cell percentage in apoptosis from three independent experiments (**, p < 0.01)