Fig. 2

Histological findings of the vaginal malignant melanoma biopsied on the first day (before itraconazole treatment). a and b Haematoxylin and eosin staining. a Nested tumour cells can be observed under the normal squamous epithelium lining; the tumour cells show pleomorphism, multinuclei, bizarre nuclei, prominent nucleoli, and mitosis (inset). b Some tumour cells contain fine melanin granules. c Immunostaining with anti-Melan A antibody; most tumour cells shows strong cytoplasmic staining with anti-Melan A antibody. d Immunostaining with programmed death-ligand 1 (PD-L1) antibody (28–8) shows membranous and cytoplasmic staining in 20% of tumour cells