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Table 1 Primary and secondary antibodies/kits used for immunohistochemistry

From: Effects of indole-3-carbinol on steroid hormone profile and tumor progression in a mice model of canine inflammatory mammarycancer

Primary Antibody

Type

Source

Incubation

Secondary Antibody/kit

Source

Incubation

Ki-67 clon MIB-1

(Ref. M7240)

Mab

Dako

1:75

60 min, RT

EnVision+SystemHRP (DAB)

(Ref. K4007)

Dako

kit

40 min, RT

ERα clon 1D5

(Ref. M7047)

Mab

Dako

1:15

Overnight

4 ° C

EnVision + SystemHRP (DAB)

(Ref. K4007)

Dako

kit

40 min, RT

Caspase-3

(Ref. AF835)

Pab

R&D Systems

1:1200

Overnight

4 ° C

Swine anti-rabbit biotinylated

(Ref. E0353)

Dako 1:200

30 min, RT

  1. Mab mouse monoclonal antibody, Pab rabbit polyclonal antibody