Primary Antibody | Type | Source | Incubation | Secondary Antibody/kit | Source | Incubation |
---|---|---|---|---|---|---|
Ki-67 clon MIB-1 (Ref. M7240) | Mab | Dako 1:75 | 60Â min, RT | EnVision+SystemHRP (DAB) (Ref. K4007) | Dako kit | 40Â min, RT |
ERα clon 1D5 (Ref. M7047) | Mab | Dako 1:15 | Overnight 4 ° C | EnVision + SystemHRP (DAB) (Ref. K4007) | Dako kit | 40 min, RT |
Caspase-3 (Ref. AF835) | Pab | R&D Systems 1:1200 | Overnight 4 ° C | Swine anti-rabbit biotinylated (Ref. E0353) | Dako 1:200 | 30 min, RT |