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Fig. 5 | BMC Cancer

Fig. 5

From: ARL2 overexpression inhibits glioma proliferation and tumorigenicity via down-regulating AXL

Fig. 5

AXL overexpression partially restores the phenotype change induced by ARL2 overexpression. a qPCR analyses of AXL in U251 cells transduced with lentiviral ARL2 overexpression vector together with AXL overexpression or control vector. (P < 0.0001, n = 3, t test). b Western blot images and analyses of AXL in U251 glioma cells transduced with lentiviral ARL2 overexpression vector together with AXL overexpression or control vector. (P < 0.0001, n = 3, t test). c In vitro growth assay showed that the inhibition of U251 proliferation induced by ARL overexpression could be partly rescued by AXL overexpression. (Day 2,P = 0.0055;Day 4,P < 0.0001; Day 6, P < 0.0001; n = 6, t test). d Colony formation assay revealed that the inhibition of clone formation capabilities in U251 cells induced by ARL2 overexpression could be partially rescued by AXL overexpression. (1000 cells/well, P < 0.001, n = 3, t test). e Wound healing test showed that the inhibition of migration capabilities in U251 cells induced by ARL2 overexpression could be partially rescued by AXL overexpression. (P < 0.01, n = 3, t test). f Transwell assay confirmed that AXL overexpression can partially rescued the invasion capability inhibition in U251 cells induced by ARL2 overexpression. Scale bar, 100 μm. (P < 0.01, n = 3, t test). g The diagram illustrated that ARL2 up-regulation decreased the capabilities of proliferation, invasion and tumorigenesis via inhibiting the expression of AXL in glioma cells

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