Fig. 4

ARL2 decreases AXL expression in glioma cells. a GSEA analysis showed that the expression of ARL2 is associated with substrate adhesion dependent cell spreading signaling pathway (33 genes were enriched and AXL was involved, http://www.broadinstitute.org/gsea/index.jsp). The normalized enrichment scores (NES) and the p values are shown in the plot. b, c Representative images and analysis of western blot demonstrated that ARL2 overexpression decreased AXL protein expression in U251 (b) and U87 (c) cells. (U251, P = 0.038, n = 3; U87, P = 0.0053; n = 4, t test). d Representative images of immunocytochemistry showed that ARL2 overexpression decreased AXL expression. e The IHC staining of U87 xenograft confirmed that AXL expression decreased after ARL2 overexpression. Scale bar = 100 μm. f ARL2 overexpression decreased phospho-AXL protein expression in U251 cells (P = 0.0018, n = 3, t test). g ARL2 overexpression inhibited phospho-ERK expression (total ERK, P = 0.9975; phospho-ERK, P = 0.0019, n = 4, t test)