Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: ARL2 overexpression inhibits glioma proliferation and tumorigenicity via down-regulating AXL

Fig. 2

ARL2 regulated the growth, colony formation, migration and invasion capabilities of glioma cells. a, b The representative western blot images and analyses of ARL2 in U251 (a) and U87 (b) glioma cells transduced with lentiviral ARL2 vector or its control vector (U251, P = 0.0099; U87, P = 0.0316; n = 3; t test). c, d In vitro growth assay showed that ARL2 overexpression inhibited the proliferation of U251 (c) and U87 (d) (** P < 0.01; ****P < 0.0001; n = 5, t test). e, f Colony formation assay revealed that ARL2 overexpression reduces clone formation ability of U251 (e) and U87 (f) cell lines (500 cells/well; U251, P = 0.0004; U87, P = 0.0014; n = 3, t test). g, h Wound healing test showed that ARL2 overexpression decreased the migration abilities of U251 (g) and U87 (h) cells (U251, P = 0.0111; U87, P = 0.0015; n = 3, t test). i, j Transwell assay demonstrated that ARL2 overexpression inhibited the invasion capabilities of U251 (i) and U87 (j) cells. Scale bar, 100 μm. (P < 0.01, n = 3, with t test)

Back to article page