Fig. 5

Immunofluorescence (a) and Western blot analysis (b) of CBS, CSE and MPST in control RCC4 cells (cont) and cells with induced apoptosis (AIK). In group of AIK, rapid upregulation mainly of CBS and CSE was detectable (a, green signal). Signal of MPST seems to be not increased so much. Nuclei were counterstained with DAPI (blue signal). Scale bar represents 100 μM. Upregulation of CBS, CSE and MPST observed by immunofluorescence was verified by Western blot analysis (b). All three enzymes were upregulated due to AIK treatment