Fig. 4

IKKε inhibits p52 activity independent of interactions with NIK and IKKα. a) Binding activity of the NF-κB p52 transcription factor was significantly decreased in the presence of IKKε. b) siRNA-mediated knockdown of NFKB2 resulted in a significant decrease of CD44 and CXCL1 mRNA expression. c) siRNA-mediated knockdown of IKBKE resulted in a significant increase in mRNA expression of RELB, NFKB2, and CXCL1. d) CHiP-PCR experiments demonstrate significant enrichment of p52 at the promoter of CXCL1 when IKKε is knocked down. e) Co-immunoprecipitation experiments indicate IKKε does not interact with NIK or IKKα. * significantly different from corresponding shNeg or siNeg control, P < 0.05, two-sided unpaired t-test