Fig. 5

Autophagy induced by RSV in HL-60 cells depends on the PI3K-AKT and LKB1-AMPK-mTOR pathways. a HL-60 cells were treated with different concentrations of RSV for 24 h, or treated with 50 μM RSV for different times, then PI3K(P85), p-AKT/AKT, p-LKB1/LKB1, p-Raptor/Raptor and p-p70S6K/p70S6K were analyzed by western-blot. β-Actin was used as an internal control to show equal amounts of protein were applied. b HL-60 cells were incubated with 50 μM RSV combined with compound C (1 mM) for 24 h, then P-AMPK, P-P70S6K, LC3 and cleaved caspase3 were analyzed by western blot analysis with the corresponding antibodies. β-Actin was used as the loading control. Data are presented as mean ± S.D. of three independent experiments in duplicates. *p < 0.05, vs. control